Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. 5 Oxoprolinuria as a Cause for Metabolic Acidosis Renal Fellow Network Myth busting, Acetaminophen version. "Avoid Acetaminophen (Tylenol) because it causes glutathione depletion". Truth?? As a pediatrician and ER doctor and as moms we're often asked about this. And we understand why: About Acetaminophen Overdose Acetaminophen Overdose Reveals PAR4 as a Low Expressing but Potent Receptor on the Hepatic Endothelium in Mice Arteriosclerosis, Thrombosis, and Vascular Biology Acetylcysteine for Acetaminophen Poisoning New England Journal of Medicine